ACHIEVEMENTS

2024

• Kawakami T, Tomita H, Hien PT, Matsumoto K (2024) Biosynthesis of high toughness poly(3-hydroxypropionate)-based block copolymers with poly(D-2-hydroxybutyrate) and poly(D-lactate) segments using evolved monomer sequence-regulating polyester synthase. Biopolymers 115(6):e23618.
• Takafuji Y, Fischer T, Miyazaki K, and Honda K. (2024) Complete genome sequences of Thermus thermophilusstrains isolated from Shirahama Hot Spring in Japan. Microbiol Res Announc 13(6):e00316-24
• Imai T, Naruse M, Horikawa Y, Yaoi K, Miyazaki K, Sugiyama J (2024) Disturbance of the hydrogen bonding in cellulose by bacterial expansin. Cellolose 30:8423–8438
• Phothong N, Pattarakankul T, Morikane S, Plaga T, Aht-Ong D, Honda K, Napathorn SC (2024) pH-Responsive polyhydroxybutyrate/cellulose acetate phthalate microbeads loaded with the water-soluble bioactive compound niacinamide. Int. J Biol Macromol 271(2):132680
• Hozumi Y, Hachisuka SI, Tomita H, Kikukawa H, Matsumoto K (2024) Engineering of the long-main-chain monomer-incorporating polyhydroxyalkanoate synthase PhaC_AR for the biosynthesis of poly[(R)-3-hydroxybutyrate-co-6-hydroxyhexanoate]. Biomacromolecules 25(5):2973-2979.
• Tanaka T, Sugiyama R, Sato Y, Kawaguchi M, Honda K, Iwaki H, Okano K (2024) Precise microbiome engineering using natural and synthetic bacteriophages targeting an artificial bacterial consortium. Front Microbiol 15:1403903
• Sato Y, Okano K, Honda K (2024) Effects of small heat shock proteins from thermotolerant bacteria on the stress resistance of Escherichia colito temperature, pH, and hyperosmolarity. Extremophiles 28(1):12
• Hizume T, Sato Y, Iwaki H, Honda K, Okano K (2024) Subtractive modification of bacterial consortium using antisense peptide nucleic acids. Front Microbiol 14:1321428
• Tong CY, Honda K, Derek CJC (2024) Enhancing organic matter productivity in microalgal-bacterial biofilm using novel bio-coating. Sci Total Environ 906:167576
• Tong CY, Honda K, Derek CJC (2024) Enhanced microalgal growth and metabolites through co-cultivation with Escherichia coliin algal organic matter solution. Algal Res 77:103351
• 宮崎健太郎 (2024) 「rRNAの進化と進化工学」 RNAの科学 時代を拓く生体分子（朝倉書店）

2023

• Tong CY, Kee CY, Honda K, Derek CJC (2023) Bio-coatings in permeated cultivation systems: Unprecedented impacts on microalgal monoculture growth and organic matter yield. Environ Res 239(2):117403
• Suryatin Alim G, Suzuki T, Honda K (2023) Cell-free production and regeneration of cofactors. Adv Biochem Eng Biotechnol 186:29-49.
• Kato S, Takeuchi K, Iwaki M, Miyazaki K, Honda K, Hayashi T (2023) Chitin- and streptavidin-mediated affinity purification systems: A screening platform for enzyme discovery. Angew Chem Int Ed 62(31):e202303764
• Hien PT, Furukawa S, Imai K, Tomita H, Isono T, Satoh T, Matsumoto K (2023) Biosynthesis of high-molecular-weight poly(D-lactate)-containing block copolyesters using evolved sequence-regulating polyhydroxyalkanoate synthase PhaC_AR. ACS Sustain Chem Eng 11(30):11123-11129.
• Tong CY, Honda K, Derek CJC (2023) A review on microalgal-bacterial co-culture: The multifaceted role of beneficial bacteria towards enhancement of microalgal metabolite production. Environ Res 228:115872
• Wu C-C, Honda K, Fujiyama K (2023) Current advances in alteration of fatty acid profile in Rhodotorula toruloides: a mini-review. World J Microbiol Biotechnol 39(9):234
• Yanagawa K, Kajikawa A, Sakakibara S, Kumeta H, Tomita H, Matsumoto K (2023) Real-time NMR analysis of polyhydroxyalkanoate synthase reaction that synthesizes block copolymer comprising glycolate and 3-hydroxybutyrate. Biophys Chem 296:107001.
• Sinsukudomchai P, Aht-Ong D, Honda K, Napathorn SC (2023) Green composites made of polyhydroxybutyrate and long-chain fatty acid esterified microcrystalline cellulose from pineapple leaf. Plos One 18(3):e0282311
• Sakuma M, Honda S, Ueno H, Tabata VK, Miyazaki K, Tokuriki N, Noji H (2023) Genetic perturbation alters functional substates in alkaline phosphatase. J Am Chem Soc 145(5):2806-2814
• 佐藤悠, 本田孝祐, 宮崎健太郎 (2023) 「極限環境微生物の探索と解析」極限環境微生物の先端科学と社会実装最前線（エヌ・ティー・エス）

2022

• Yakabe K, Higashi S, Akiyama M, Mori H, Murakami T, Toyoda A, Sugiyama Y, Kishino S, Okano K, Hirayama A, Gotoh A, Li S, Mori T, Katayama T, Ogawa J, Fukuda S, Hase K, Kim YG (2022) Dietary-protein sources modulate host susceptibility to Clostridioides difficile infection through the gut microbiota. Cell Rep 40(11):111332
• Suryatin Alim G, Okano K, Honda K (2022) One-step preparation of cell-free ATP regeneration module based on non-oxidative glycolysis using thermophilic enzymes. ChemBioChem 23(16):e202200210
• Miyazaki K (2022) Complete genome sequences of Thermus strains isolated from Senami Hot Spring in Japan. Microbiol Resour Announc 11(7):e00354-22
• Miyazaki K, Hosoya K (2022) Complete genome sequences of four Parageobacillus strains isolated from soil in Japan. Microbiol Resour Announc 11(6):e00204-22
• Okano K, Sato Y, Hama S, Tanaka T, Noda H, Kondo A, Honda K (2022) L-Lactate oxidase-mediated removal of L-lactic acid derived from fermentation medium for the production of optically pure D-lactic acid. Biotechnol J 17(4):e2100331
• Ma’ruf IF, Restiawaty E, Syihab SF, Honda K, Akhmaloka (2022) Characterization of thermostable serine hydroxymethyltransferase for β-hydroxy amino acids synthesis. Amino Acids 55(1):75-88

2021

• Matsui N, Kawakami S, Hamamoto D, Nohara S, Sunada R, Panbangred W, Igarashi Y, Nihira T, Kitani S (2021) Activation of cryptic milbemycin A₄production in Streptomyces BB47 by the introduction of a functional bldA gene. J Gen Appl Microbiol 67(6):240-247       休眠抗生物質の生産が覚醒した放線菌の写真が表紙を飾りました
• Imura M, Etoh S, Iwakiri R, Okano K, Honda K (2021) Improvement of production yield of L-cysteine through in vitrometabolic pathway with thermophilic enzymes. J Biosci Bioeng 132(6):585-591
• Okano K, Sato Y, Hizume T, Honda K (2021) Genome editing by miniature CRISPR/Cas12f1 enzyme in Escherichia coli. J Biosci Bioeng 132(2):120-124
• Ma’ruf IF, Sasaki Y, Kerbs A, Nießer J, Sato Y, Taniguchi H, Okano K, Kitani S, Restiawaty E, Akhmaloka, Honda K (2021) Heterologous gene expression and characterization of two serine hydroxymethyltransferases from Thermoplasma acidophilum. Extremophiles 25(4):393-402
• Wu C-C, Ohashi T, Kajiura H, Sato Y, Misaki R, Honda K, Limtong S, Fujiyama K (2021) Functional characterization and overexpression of Δ12-desaturase in the oleaginous yeast Rhodotorula toruloidesfor production of linoleic acid-rich lipids. J Biosci Bioeng 131(6):631-639
• Suryatin Alim G, Iwatani T, Okano K, Kitani S, Honda K (2021) In vitro production of coenzyme A using thermophilic enzymes. Appl Environ Microbiol 87(14):e0054121
• Boontip T, Waditee-Sirisattha R, Honda K, Napathorn SC (2021) Strategies for poly (3-hydroxybutyrate) production using a cold-shock promoter in Escherichia coli. Front Bioeng Biotechnol 9:666036
• Yoshioka T, Igarashi Y, Namba T, Ueda S, Pait IGU, Nihira T Kitani S (2021) Lavencidin, a polyene macrolide antibiotic from Streptomyces lavendulaeFRI-5. J Antibiot 74(5):359-362
• Okazawa A, Samejima H, Kitani S, Sugimoto Y, Ohta D (2021) Germination stimulatory activity of bacterial butenolide hormones from Streptomyces albusJ1074 on seeds of the root parasitic weed Orobanche minor. J Pestic Sci 46(2):242-247
• Napathorn S C, Visetkoop S, Pinyakong O, Okano K, Honda K (2021) Polyhydroxybutyrate (PHB) production using an arabinose-inducible expression system in Escherichia coli. Front Bioeng Biotechnol 9:661096
• Dissook S, Kuzuyama T, Nishimoto Y, Kitani S, Putri S, Fukusaki E (2021) Stable isotope and chemical inhibition analyses suggested the existence of a non-mevalonate-like pathway in the yeast Yarrowia lipolytica. Sci Rep 11(1):5598
• 本田孝祐 (2021) 「つくって利用する生物学」の実践～分子微生物学研究室の紹介～, 生産と技術, 73: 32-35

2020

• Okano K, Sato Y, Inoue S, Kawakami S, Kitani S, Honda K (2020) Enhancement of S-adenosylmethionine-dependent methylation by integrating methanol metabolism with 5-methyltetrahydrofolate formation in Escherichia coli. Catalysts 10(9):1001
• Sato Y, Okano K, Kimura H, Honda K (2020) TEMPURA: Database of growth TEMPeratures of Usual and RAre prokaryotes. Microbes Environ 35(3):ME20074
• Okano K, Zhu Q, Honda K (2020) In vitro reconstitution of non-phosphorylative Entner–Doudoroff pathway for lactate production. J Biosci Bioeng 129(3):269-275
• 本田孝祐, 岡野憲司 (2020) 好熱性酵素を用いた細胞外カスケード反応の構築と有用物質生産への利用　細胞の外側でつくる人工代謝経路, 化学と生物, 58: 389-395
• 木谷茂 (2020) 抗生物質生産微生物における潜在代謝能力の活性化, 生産と技術, 72:81-83

2019

• Ogawa M, Uyeda A, Harada K, Sato Y, Kato Y, Watanabe H, Honda K, Matsuura T (2019) Class III polyphosphate kinase 2 enzymes catalyze the pyrophosphorylation of adenosine-5′-monophosphate. ChemBioChem 20(23):2961-2967
• Hanatani Y, Imura M, Taniguchi H, Okano K, Toya Y, Iwakiri R, Honda K (2019) In vitro production of cysteine from glucose. Appl Microbiol Biotechnol 103(19):8009–8019
• Taniguchi H, Imura M, Okano K, Honda K (2019) Developing a single strain for in vitro salvage synthesis of NAD+ at high temperatures and its potential for bioconversion. Microb Cell Fact 18(1):75
• Honda K, Ono T, Okano K, Miyake R, Dekishima Y, Kawabata H (2019) Expression of engineered carbonyl reductase from Ogataea minutain Rhodococcus opacus and its application to whole-cell bioconversion in anhydrous solvents. J Biosci Bioeng 127(2):145-149
• Ueda S, Ikeda H, Namba T, Ikejiri Y, Nihimoto Y, Arai M, Nihira T, Kitani S (2019) Identification of biosynthetic genes for the β-carboline alkaloid kitasetaline and production of the fluorinated derivatives by heterologous expression. J Ind Microbiol Biotechnol 46(5):739-750