ACHIEVEMENTS

2024

Takafuji Y, Fischer T, Miyazaki K, and Honda K. (2024)     https://journals.asm.org/doi/10.1128/mra.00316-24
Complete genome sequences of Thermus thermophilus strains isolated from Shirahama Hot Spring in Japan. Microbiol. Res. Announc. 13(6):e00316-24
  • Phothong, N., Pattarakankul, T., Morikane, S., Plaga, T., Aht-Ong, D., Honda, K., Napathorn, S. C. (2024) pH-Responsive polyhydroxybutyrate/cellulose acetate phthalate microbeads loaded with the water-soluble bioactive compound niacinamide. Int. J. Biol. Macromol. In Press
  • Tanaka, T., Sugiyama, R., Sato, Y., Kawaguchi, M., Honda, K., Iwaki, H., Okano, K. (2024) Precise microbiome engineering using natural and synthetic bacteriophages targeting an artificial bacterial consortium. Front. Microbiol., 15: 1403903
  • Sato, Y., Okano, K., Honda, K. (2024) Effects of small heat shock proteins from thermotolerant bacteria on the stress resistance of Escherichia coli to temperature, pH, and hyperosmolarity. Extremophiles, 28: 12
  • Hizume, T., Sato, Y., Iwaki, H., Honda, K., Okano, K. (2024) Subtractive modification of bacterial consortium using antisense peptide nucleic acids. Front. Microbiol., 14: 1321428
  • Tong, C.Y., Honda, K., Derek, C.J.C. (2024) Enhancing Organic Matter Productivity in Microalgal-bacterial Biofilm using Novel Bio-coating. Sci. Total Environ., 906: 167576
  • Tong, C.Y., Honda, K., Derek, C.J.C. (2024) Enhanced microalgal growth and metabolites through co-cultivation with Escherichia coli in algal organic matter solution. Algal Res., 77: 103351

2023

  • Tong, C.Y., Kee, C.Y., Honda, K., Derek, C.J.C. (2023) Bio-coatings in permeated cultivation systems: Unprecedented impacts on microalgal monoculture growth and organic matter yield. Environ. Res., 239: 117403
  • Suryatin Alim, G., Suzuki, T., Honda, K. (2023) Cell-Free Production and Regeneration of Cofactors. In: Advances in Biochemical Engineering/Biotechnology, Springer, Berlin, Heidelberg., doi.org/10.1007/10_2023_222
  • Tong, C.Y., Honda, K., Derek, C.J.C. (2023) A review on microalgal-bacterial co-culture: The multifaceted role of beneficial bacteria towards enhancement of microalgal metabolite production. Environ. Res., 228: 115872
  • Kato, S., Takeuchi, K., Iwaki, M., Miyazaki, K., Honda, K., Hayashi, T. (2023) Chitin- and Streptavidin-Mediated Affinity Purification Systems: A Screening Platform for Enzyme Discovery. Angew. Chem., doi.org/10.1002/ange.202303764
  • Wu, C.-C., Honda, K., Fujiyama, K. (2023) Current advances in alteration of fatty acid profile in Rhodotorula toruloides: a mini-review. World J. Microbiol. Biotechnol., 39:234
  • Sinsukudomchai, P., Aht-Ong, D., Honda, K., Napathorn, S.C. (2023) Green composites made of polyhydroxybutyrate and long-chain fatty acid esterified microcrystalline cellulose from pineapple leaf. Plos One 18: e0282311
  • Sakuma, M., Honda, S., Ueno, H., Tabata V. K., Miyazaki, K., Tokuriki, N., Noji, H. (2023) Genetic perturbation alters functional substates in alkaline phosphatase. J. Am. Chem. Soc., doi: 10.1021/jacs.2c06693

2022

  • Ma’ruf, I. F., Restiawaty, E., Syihab, S. F., Honda, K., Akhmaloka (2022) Characterization of thermostable serine hydroxymethyltransferase for β-hydroxy amino acids synthesis. Amino Acids, 55: 75-88
  • Yakabe, K., Higashi, S., Akiyama, M., Mori, H., Murakami, T., Toyoda, A., Sugiyama, Y., Kishino, S., Okano, K., Hirayama, A., Gotoh, A., Li, S., Mori, T., Katayama, T., Ogawa, J., Fukuda, S., Hase, K., Kim, Y.G. (2022) Dietary-protein sources modulate host susceptibility to Clostridioides difficile infection through the gut microbiota. Cell Rep., 40: 111332
  • Suryatin Alim, G., Okano, K., Honda, K. (2022) One-step preparation of cell-free ATP regeneration module based on non-oxidative glycolysis using thermophilic enzymes. ChemBioChem, 23: e202200210
  • Miyazaki, K. (2022) Complete Genome Sequences of Thermus Strains Isolated from Senami Hot Spring in Japan. Microbiol. Resour., 11: e00354-22
  • Miyazaki, K., Hosoya K. (2022) Complete Genome Sequences of Four Parageobacillus Strains Isolated from Soil in Japan. Microbiol. Resour., 11: e00204-22
  • Okano, K., Sato, Y., Hama, S., Tanaka, T., Noda, H., Kondo, A., Honda, K. (2022) L-Lactate oxidase-mediated removal of L-lactic acid derived from fermentation medium for the production of optically pure D-lactic acid. Biotechnol. J., 17: e2100331

2021

  • Matsui M., Kawakami S., Hamamoto D., Nohara S., Sunada R., Panbangred W., Igarashi Y., Nihira T., and Kitani S. (2021) Activation of cryptic milbemycin A4 production in Streptomyces sp. BB47 by the introduction of a functional bldA gene. J. Gen. Appl. Microbiol., 67: 240-247       休眠抗生物質の生産が覚醒した放線菌の写真が表紙を飾りました
  • Okazawa A., Samejima H., Kitani S., Sugimoto Y., and Ohta D. (2021) Germination stimulatory activity of bacterial butenolide hormones from Streptomyces albus J1074 on seeds of the root parasitic weed Orobanche minor . J. Pestic. Sci., 46: 242-247
  • Yoshioka T., Igarashi Y., Namba T., Ueda S., Pait IGU., Nihira T. and Kitani S. (2021) Lavencidin, a polyene macrolide antibiotic from Streptomyces lavendulae FRI-5. J. Antibiot. (Tokyo)., 74: 359-362
  • Imura, M., Etoh, S., Iwakiri, R., Okano, K., Honda, K. (2021) Improvement of production yield of L-cysteine through in vitro metabolic pathway with thermophilic enzymes. J. Biosci. Bioeng., 132: 585-591
  • Ma’ruf, I. F., Sasaki, Y., Kerbs, A., Nießer, J., Sato, Y., Taniguchi, H., Okano, K., Kitani, S., Restiawaty, E., Akhmaloka, Honda, K. (2021) Heterologous gene expression and characterization of two serine hydroxymethyltransferases from Thermoplasma acidophilum. Extremophiles, 25: 393-402
  • Boontip, T., Waditee-Sirisattha, R., Honda, K., Napathorn, S.C. (2021) Strategies for poly (3-hydroxybutyrate) production using a cold-shock promoter in Escherichia coli. Front. Bioeng. Biotechnol., 9: 666036
  • Suryatin Alim, G., Iwatani, T., Okano, K., Kitani, S., Honda, K. (2021) In vitro production of coenzyme A using thermophilic enzymes. Appl. Environ. Microbiol. AEM. 00541-21
  • Okano, K., Sato, Y., Hizume, T., Honda, K. (2021) Genome editing by miniature CRISPR/Cas12f1 enzyme in Escherichia coli. J. Biosci. Bioeng., 132: 120-124
  • Dissook, S., Kuzuyama, T., Nishimoto, Y., Kitani, S., Putri, S., Fukusaki, E. (2021) Stable isotope and chemical inhibition analyses suggested the existence of a non-mevalonate-like pathway in the yeast Yarrowia lipolytica. Sci Rep., 11: 5598
  • Napathorn, S. C., Visetkoop, S., Pinyakong, O., Okano, K., Honda, K. (2021) Polyhydroxybutyrate (PHB) production using an arabinose-inducible expression system in Escherichia coli. Front. Bioeng. Biotechnol. 9: 661096
  • Wu C.-C., Ohashi, T., Kajiura, H., Sato, Y., Misaki, R., Honda, K., Limtong, S., Fujiyama, K. (2021) Functional characterization and overexpression of Δ12-desaturase in the oleaginous yeast Rhodotorula toruloides for production of linoleic acid-rich lipids. J. Biosci. Bioeng., 131: 631-639
  • 本田孝祐 (2021) 「つくって利用する生物学」の実践~分子微生物学研究室の紹介~, 生産と技術, 73: 32-35

2020

  • 木谷茂 (2020) 抗生物質生産微生物における潜在代謝能力の活性化, 生産と技術, 72: 81-83
  • Okano, K., Sato, Y., Inoue, S., Kawakami, S., Kitani, S., Honda, K. (2020) Enhancement of S-adenosylmethionine-dependent methylation by integrating methanol metabolism with 5-methyltetrahydrofolate formation in Escherichia coli. Catalysts, 10: 1001
  • Sato, Y., Okano, K., Kimura, H., Honda, K. (2020) TEMPURA: Database of growth TEMPeratures of Usual and RAre prokaryotes. Microbes Environ. 35:ME20074
  • 本田孝祐, 岡野憲司 (2020) 好熱性酵素を用いた細胞外カスケード反応の構築と有用物質生産への利用 細胞の外側でつくる人工代謝経路, 化学と生物, 58: 389-395
  • Okano, K., Zhu, Q., Honda, K. (2020) In vitro reconstitution of non-phosphorylative Entner–Doudoroff pathway for lactate production. J. Biosci. Bioeng., 129: 269-275

2019

  • Ogawa, M., Uyeda, A., Harada, K., Sato, Y., Kato, Y., Watanabe, H., Honda, K., Matsuura, T. (2019) Class III polyphosphate kinase 2 enzymes catalyze the pyrophosphorylation of adenosine-5′-monophosphate. ChemBioChem, 20: 2961-2967
  • Hanatani, Y., Imura, M., Taniguchi, H., Okano, K., Toya, Y., Iwakiri, R., Honda, K. (2019) In vitro production of cysteine from glucose. Appl. Microbiol. Biotechnol., 103: 8009–8019
  • Taniguchi, H., Imura, M., Okano, K., Honda, K. (2019) Developing a single strain for in vitro salvage synthesis of NAD+ at high temperatures and its potential for bioconversion. Microb. Cell Fact., 18: 75
  • Honda, K., Ono, T., Okano, K., Miyake, R., Dekishima, Y., Kawabata, H. (2019) Expression of engineered carbonyl reductase from Ogataea minuta in Rhodococcus opacus and its application to whole-cell bioconversion in anhydrous solvents. J. Biosci. Bioeng., 127: 145-149
  • Ueda, S., Ikeda, H., Namba, T., Ikejiri, Y., Nihimoto, Y., Arai, M., Nihira, T., Kitani, S. (2019) Identification of biosynthetic genes for the β-carboline alkaloid kitasetaline and production of the fluorinated derivatives by heterologous expression. J. Ind. Microbiol. Biotechnol. 46: 739-750

THE HONDA LABORATORY

Laboratory of Molecular Microbiology,
International Center for Biotechnology (ICBiotech), Osaka University

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